复方中药对猪嗜血支原体感染预防作用的研究

doi:10.11843/j.issn.0366-6964.2016.02.026

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (2): 404-410.doi: 10.11843/j.issn.0366-6964.2016.02.026

复方中药对猪嗜血支原体感染预防作用的研究

董耀泽，李晓云，张晋强，石泽宇，陈建闪，李宏全，马海利^*

（山西农业大学动物科技学院，太谷 030801）

收稿日期:2015-07-13 出版日期:2016-02-23 发布日期:2016-02-23
通讯作者: 马海利，教授，E-mail：mahaili1718@126.com
作者简介:董耀泽（1989-），男，山西太原人，硕士，主要从事动物传染病的诊断与防治的研究，E-mail：t123o456m789@163.com
基金资助:
山西省科技攻关项目(20130311028-1)

Prophylactic Effect of Compound Chinese Herbal Medicine on Mycoplasma suis Infection

DONG Yao-ze，LI Xiao-yun，ZHANG Jin-qiang，SHI Ze-yu，CHEN Jian-shan，LI Hong-quan，MA Hai-li^*

(College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China)

Received:2015-07-13 Online:2016-02-23 Published:2016-02-23

摘要/Abstract

摘要：

为研究复方中药对猪嗜血支原体（M.suis）感染的预防效果，将复方中药Ⅰ、Ⅱ、Ⅲ和强力霉素分别与M.suis阳性血液在细胞培养液中进行培养，间隔12 h用qPCR检测培养物中M.suis拷贝数。选取临床健康的35日龄仔猪进行M.suis PCR检测，将50头M.suis PCR阳性仔猪随机分为5组，每组10头，每组隔离饲养。三个复方中药组采用拌料方式连续喂服7 d，强力霉素组拌料喂服5 d，对照组饲料中不添加任何药物。分别于用药前、用药后第10、20、30天前腔静脉采血，对血液M.suis拷贝数、红细胞SOD活力、红细胞膜ATP酶（Na⁺K⁺-ATPase、Ca²⁺Mg²⁺-ATPase）活力、淋巴细胞（T、B）增殖率、血清IgG含量进行检测。结果显示，三种复方中药在体外培养时均可极显著降低培养物中M.suis拷贝数（P＜0.01）；在仔猪预防试验中，与对照组和强力霉素组相比，复方中药Ⅰ可以极显著降低血液中M.suis拷贝数（P＜0.01），提高红细胞SOD活力与B淋巴细胞增殖率（P＜0.05或P＜0.01）；复方中药Ⅱ可以提高红细胞SOD活力、Na⁺K⁺-ATPase活力与淋巴细胞增殖率（P＜0.05），但无法长时间抑制M.suis生长；复方中药Ⅲ可以极显著降低血液中M.suis拷贝数（P＜0.01），显著提高红细胞SOD活力（P＜0.05）。结果说明：复方中药Ⅰ对M.suis自然感染有良好的预防作用。

Abstract:

The study aimed to develop compound Chinese herbal medicine that is more efficient in prevention and control of the M.suis infection.M.suis of positive blood were cultured in cell culture fluid with the compound Chinese herbal medicine Ⅰ，Ⅱ，Ⅲ and deoxytetracycline.The copies of M.suis in cell culture fluid were measured by qPCR every 12 h.Fifty clinical healthy，35 day-old piglets with natural infection of M.suis confirmed by PCR were randomly allocated to 5 groups，each with 10 replicates，and all the groups were fed separately.Piglets in three compound Chinese herbal medicine group were fed with a diet that contained 20 g•kg^-1 of the compound Chinese herbal medicine Ⅰ，Ⅱ，and Ⅲ respectively for seven days；piglets in deoxytetracycline group were fed with a diet that mixed 200 mg•kg^-1 of deoxytetracycline for five days；piglets in control group were fed with diet.Blood samples of piglets were collected by the anterior vena before (0 day) and after the usage of medicine at the 10^th，20^thand 30^th days.The copies of M.suis in blood，erythrocyte superoxide dismutase，erythrocyte membrane ATPase (Na⁺K⁺-ATPase，Ca²⁺Mg²⁺-ATPase)，lymphocyte proliferation rate (T，B)，and the serum content of IgG were measured.The results showed that：the three compound Chinese herbal medicine could significantly decrease the copies of M.suis in vitro (P＜0.01)；compared with the deoxytetracycline group and the control group，the compound Chinese herbal medicine Ⅰ could significantly decrease the copies of M.suis in blood of piglets (P＜0.01)，improve the activity of erythrocyte superoxide dismutase and B-proliferation rate (P＜0.05)；the compound Chinese herbal medicine Ⅱ had positive effects on erythrocyte superoxide dismutase，the activity of erythrocyte membrane Na⁺K⁺-ATPase and lymphocyte proliferation rate (P＜0.05)，whereas it could not effectively reduce the M.suis copies；the compound Chinese medicine Ⅲ could reduce the M.suis copies in blood of piglets (P＜0.01) and only improve the activity of erythrocyte superoxide dismutase (P＜0.05).In conclusion，the compound Chinese medicineⅠhas favorable prophylactic effects on the M.suis infection.

中图分类号:

董耀泽，李晓云，张晋强，石泽宇，陈建闪，李宏全，马海利. 复方中药对猪嗜血支原体感染预防作用的研究[J]. 畜牧兽医学报, 2016, 47(2): 404-410.

DONG Yao-ze，LI Xiao-yun，ZHANG Jin-qiang，SHI Ze-yu，CHEN Jian-shan，LI Hong-quan，MA Hai-li. Prophylactic Effect of Compound Chinese Herbal Medicine on Mycoplasma suis Infection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 404-410.

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复方中药对猪嗜血支原体感染预防作用的研究

Prophylactic Effect of Compound Chinese Herbal Medicine on Mycoplasma suis Infection

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